Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported (for relevant formats) applications include: Western Blot, blocking function, immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
Application References:
1. Takakura N, et al. 1996. J Invest Dermatol. 107:770
Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
C57BL/6 splenocytes stained with APA5 PE
Description:
Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic
tissues and mesenchymal-derived cells of adult mouse. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Other Names:
PDGF receptor-α, PDGFR-α
Structure:
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution:
Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function:
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand Receptor:
PDGFs
Antigen References:
1. Mukouyama, Y.S. et al. 2006. Proc Natl Acad Sci USA. 103(5): 1551 2. Miyawaki, T. et al. 2004. J Neurosci. 24(37):8124 3. Takakura, N. et al. 1997. J Histochem Cytochem. 45(6):883
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