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Purified Anti-mouse CD140a Antibody
Material Safety Data Sheet (MSDS) Purified Anti-mouse CD140a Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-mouse CD140a Antibody
135901 25 µg $45.00     
135902 100 µg $90.00     
Clone: APA5
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application: FC - Quality tested
WB, IHC - This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported (for relevant formats) applications include: Western Blot, blocking function, immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
Application
References:
1. Takakura N, et al. 1996. J Invest Dermatol. 107:770
Mouse fibroblast cell line NIH/3T3

Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
C57BL/6 splenocytes stained with APA5

C57BL/6 splenocytes stained with APA5 PE


Description:

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic

tissues and mesenchymal-derived cells of adult mouse. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.

Other Names: PDGF receptor-α, PDGFR-α
Structure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand Receptor: PDGFs
Antigen
References:
1. Mukouyama, Y.S. et al. 2006. Proc Natl Acad Sci USA. 103(5): 1551
2. Miyawaki, T. et al. 2004. J Neurosci. 24(37):8124
3. Takakura, N. et al. 1997. J Histochem Cytochem. 45(6):883
Purified Rat IgG2a, κ Isotype CtrlRTK2758FC,ICC,IF,IHC,IP,WB
Cell Staining Buffer (FBS)FC,ICC,ICFC
RBC Lysis Buffer (10X)FC,ICFC