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Purified Anti-mouse CD140a Antibody
Material Safety Data Sheet (MSDS) Purified Anti-mouse CD140a Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-mouse CD140a Antibody
135901 25 µg $45.00     
135902 100 µg $90.00     
Clone: APA5
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application: FC - Quality tested
WB, IHC - This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported (for relevant formats) applications include: Western Blot, blocking function, immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
Application
References:
1. Takakura N, et al. 1996. J Invest Dermatol. 107:770
Mouse fibroblast cell line NIH/3T3

Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
C57BL/6 splenocytes stained with APA5

C57BL/6 splenocytes stained with APA5 PE


Description:

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic

tissues and mesenchymal-derived cells of adult mouse. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.

Other Names: PDGF receptor-α, PDGFR-α
Structure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand Receptor: PDGFs
Antigen
References:
1. Mukouyama, Y.S. et al. 2006. Proc Natl Acad Sci USA. 103(5): 1551
2. Miyawaki, T. et al. 2004. J Neurosci. 24(37):8124
3. Takakura, N. et al. 1997. J Histochem Cytochem. 45(6):883
Purified Rat IgG2a, κ Isotype CtrlRTK2758FC,ICC,IF,IHC,IP,WB
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC,ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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