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Purified anti-mouse CD138 (Syndecan-1) Antibody
Purified anti-mouse CD138 (Syndecan-1) Antibody
142501 25 µg ¥10,000     
142502 500 µg ¥35,000     

Product Details

Clone: 281-2
Isotype: Rat IgG2a, κ
Isotype Control:Purified Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and immunofluorescent staining2,3.

Application
References:

1. Jalkanen M, et al. 1985. J. Cell Biol. 101:976. (FC)
2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)

C57BL/6 mouse bone marrow cells
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.




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Description:

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Other Names: Syndecan-1
Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Antigen
References:

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell Regul. 1:27.

GeneID: 20969
Latest Publications: View the latest CD138 articles on HighwirePress.com
UniProt: View information about CD138 on UniProt.org
Keywords: Purified anti-mouse CD138 (Syndecan-1), 281-2, Purified, Syndecan-1 , Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD138 (Syndecan-1)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.





  • PE anti-mouse CD138 (Syndecan-1)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.





  • APC anti-mouse CD138 (Syndecan-1)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.





  • Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with CD19 APC and CD138 (clone 281-2) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown is from gated on lymphoid cell population.





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