Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9, (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).
Application References:
1. Granucci, F., et al., 1997. J. Immunol. 159:1794. 2. Stokes, R.W., et al., 1998. J. Immunol. 160:5514. 3. Metlay, J.P., et al., 1990. J. Exp. Med. 171:1753. (IHC, IP) 4. Ma, X.T., et al., 2006. Cancer Research 66:1169. 5. Chin, R.K., et al., 2006. J. Immunol. 177: 290. (IF) 6. Cervantes-Barragan, L., et al., 2007. Blood 109:1131. (FC) PubMed 7. Turnquist, H.R., et al., 2007. J. Immunol. 178:7018. (FC) PubMed 8. Benson, M.J., et al., 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed 9. You, Y., et al. 2009. J. Immunol. 182:7343.(IF) PubMed 10. Roland, C.L., et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed 11. Wikstrom, M., et al.2006. J. Immunol. 177:913. PubMed 12. Pericolini, E., et al. 2008. J. Leukoc. Biol. 83:1286. PubMed 13. Randall, L.M., et al. 2008. Infect. Immune.76:3312. PubMed 14. Fahlen-Yrild, L., et al. 2009. J. Immunol. 183:5032. PubMed 15. Osterholzer, J.J., et al. 2009. J. Immunol. 183:8044. PubMed 16. Bankoti, J., et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
Description:
CD11c is a 150 kD glycoprotein, also known as αX integrin, CR4, or p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands, iC3b, fibrinogen, and CD54.
Other Names:
αX integrin, integrin αX chain, CR4, p150
Structure:
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution:
dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function:
Cellular adhesion
Ligand Receptor:
iC3b, fibrinogen
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen Facts Book, Academic Press. 2. Springer, T.A. 1994. Cell 76:301. 3. Lopez-Rodriguez, C., et al., 1996. J. Immunol. 156:3780.
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