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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro blocking2 of cell-cell adhesion and immunohistochemical staining1 of acetone-fixed frozen sections. The 2D7 antibody does not block the binding of M17/4 antibody (Cat. No. 101102) to CD11a.
Application References:
1. Kootstra CJ, et al. 1997. Clin. Exp. Immunol. 108:324. (IHC) 2. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) 3. Masten BJ, et al. 1999. J. Immunol. 162:1310. (FC) 4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed 5. Cullere X, et al. 2008. Blood 111:3591. PubMed
C57BL/6 mouse splenocytes stained with purified 2D7, followed by anti-rat IgGs-FITC
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The 2D7 antibody can block CD11a-mediated cellular adhesion.
Other Names:
αL integrin, LFA-1 α subunit, Ly-15, Ly-21
Structure:
Integrin family, associates with integrin ß2 (CD18), 180 kD
Distribution:
Lymphocytes, granulocytes, monocytes, macrophages
Function:
Cellular adhesion
Ligand Receptor:
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1994. Cell 76:301. 3. Lub M, et al. 1995. Immunol. Today 16:479.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD11a
CD57BL/6 mouse splenocytes stained with 2D7 FITC
PE anti-mouse CD11a
CD57BL/6 mouse splenocytes stained with 2D7 PE
Purified anti-mouse CD11a
C57BL/6 mouse splenocytes stained with purified 2D7, followed by anti-rat IgGs-FITC
