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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2, and immunohistochemical staining3 of acetone-fixed frozen sections. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101110).
Application References:
1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block) 2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block) 3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC) 4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
C57BL/6 mouse splenocytes stained with purified M17/4, followed by anti-rat IgGs FITC
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Other Names:
αL integrin, LFA-1 α subunit, Ly-15, Ly-21
Structure:
Integrin family, associates with integrin ß2 (CD18), 180 kD
Distribution:
Lymphocytes, granulocytes, monocytes, macrophages
Function:
Cellular adhesion
Ligand Receptor:
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1994. Cell 76:301. 3. Lub M, et al. 1995. Immunol. Today 16:479.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD11a
C57BL/6 mouse splenocytes stained with biotinylated M17/4, followed by Sav-PE
FITC anti-mouse CD11a
C57BL/6 mouse splenocytes stained with M17/4 FITC
LEAF™ Purified anti-mouse CD11a
C57BL/6 mouse splenocytes stained with LEAF™ purified M17/4, followed by anti-rat IgGs FITC
PE anti-mouse CD11a
C57BL/6 mouse splenocytes stained with M17/4 PE
Purified anti-mouse CD11a
C57BL/6 mouse splenocytes stained with purified M17/4, followed by anti-rat IgGs FITC
Alexa Fluor® 488 anti-mouse CD11a
BALB/c mouse splenocytes stained with M17/4 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD11a
C57BL/6 mouse splenocytes stained with M17/4 Alexa Fluor® 647
