Antibodies
|
Search   
Purified anti-mouse CD117 (c-Kit) Antibody
Purified anti-mouse CD117 (c-Kit) Antibody
105801 50 µg $60.00 Qty:
    
105802 500 µg $195.00 Qty:
    

Product Details

 
Clone: 2B8 (See other available formats)
Isotype: Rat IgG2b, κ
Isotype Control:Purified Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse bone marrow mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of acetone fixed frozen sections2. The 2B8 antibody does not block c-Kit activity.

Application
References:

  Publication Library
Purified anti-mouse CD117 (c-Kit) Antibody 2B8 Image 1
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells.

Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand Receptor: Stem Cell Factor (SCF)
Antigen
References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

GeneID: 16590
Latest Publications: View the latest CD117 articles on HighwirePress.com
UniProt: View information about CD117 on UniProt.org
Keywords: Purified anti-mouse CD117 (c-Kit), 2B8, Purified, c-KIT, Stem Cell Factor Receptor (SCFR), Mouse, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Be the first to review this product to qualify for a $100 Amazon gift card drawing. -
See more reviews using the Reviews Library


Product FAQs

 
There are no FAQs for this product. For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
Purified anti-mouse Ly-6A/E (Sca-1)D7FC, IHC, IP, WB
Purified anti-mouse CD90G7FC, IHC
Purified anti-mouse/human CD11bM1/70FC, IHC, IF, IP, CyTOF®
Purified anti-mouse/human CD45R/B220RA3-6B2FC, IHC, IP, CyTOF®
APC Goat anti-rat IgG (minimal x-reactivity)Poly4054FC
Biotin Goat anti-rat IgG (minimal x-reactivity)Poly4054FC, ELISA, WB
FITC Goat anti-rat IgG (minimal x-reactivity)Poly4054FC
PE Goat anti-rat IgG (minimal x-reactivity)Poly4054FC
Cell Staining BufferFC, ICC, ICFC
Purified Rat IgG2b, κ Isotype CtrlRTK4530FC, ICC, ICFC, IF, IHC, IP, WB


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)2B8FC, IHC, IF
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)2B8FC
APC anti-mouse CD117 (c-Kit)2B8FC
APC/Cy7 anti-mouse CD117 (c-kit)2B8FC
Biotin anti-mouse CD117 (c-Kit)2B8FC, IHC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)2B8FC
FITC anti-mouse CD117 (c-Kit)2B8FC
Pacific Blue™ anti-mouse CD117 (c-Kit)2B8FC
PE anti-mouse CD117 (c-Kit)2B8FC
PE/Cy5 anti-mouse CD117 (c-Kit)2B8FC
PE/Cy7 anti-mouse CD117 (c-Kit)2B8FC
PerCP anti-mouse CD117 (c-kit)2B8FC
PerCP/Cy5.5 anti-mouse CD117 (c-kit)2B8FC


Related Pathways

Pathway
B cell Development and Activation
CD4 and CD8 T-Cell Lineage
Gamma-Delta T cells
Innate Immunity


Related Categories

Category
2B8
Immunology > Mouse Immunology > CD and Related Molecules > CD117 (c-kit) > 2B8
Immunology > Mouse Immunology > Stem Cells > CD117 (c-kit)

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-06-28
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD117 (c-Kit)
    C57BL/6 bone marrow cells were

    C57BL/6 bone marrow cells were stained with CD45R/B220 PE and CD117 (clone 2B8) APC (top) or rat IgG2b, κ APC isotype control (bottom).





  • Biotin anti-mouse CD117 (c-Kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with B220 APC and biotinylated CD117 (clone 2B8) or rat IgG2b, κ isotype control, followed by Sav-PE.





  • FITC anti-mouse CD117 (c-Kit)
    C57BL/6 bone marrow cells were

    C57BL/6 bone marrow cells were stained with CD45R/B220 PE and CD117 (clone 2B8) FITC (top) or rat IgG2b, κ FITC isotype control (bottom).





  • PE anti-mouse CD117 (c-Kit)
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC

  • PE/Cy5 anti-mouse CD117 (c-Kit)
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC

  • Purified anti-mouse CD117 (c-Kit)
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC

  • PE/Cy7 anti-mouse CD117 (c-Kit)
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with B220 FITC and 2B8 PE/Cy7

  • Alexa Fluor® 488 anti-mouse CD117 (c-Kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-mouse CD117 (c-Kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control

    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 647

  • Pacific Blue™ anti-mouse CD117 (c-Kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Pacific Blue™ rat IgG2b isotype control

    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Pacific Blue™

  • PerCP/Cy5.5 anti-mouse CD117 (c-kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and PerCP/Cy5.5 rat IgG2b isotype control

    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 PerCP/Cy5.5

  • PerCP anti-mouse CD117 (c-kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and PerCP rat IgG2a isotype control

    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 PerCP

  • APC/Cy7 anti-mouse CD117 (c-kit)
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with B220 (RA3-6B2) PE and 2B8 APC/Cy7

  • Brilliant Violet 421™ anti-mouse CD117 (c-Kit)
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (clone 2B8) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphoid cell population.





x
Copyright © 2014 BioLegend, Inc. All rights reserved.