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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.
Application References:
1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)
Mouse Allergin-1 transfected Ba/F3 cells were stained with purified mAb TX83 (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Allergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Allergin-1
Mouse Allergin-1 transfected Ba/F3 cells were stained with purified mAb TX83 (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
LEAF™ Purified anti-mouse Allergin-1
Mouse Allergin-1 transfected Ba/F3 cells were stained with purified mAb TX83 (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
PE anti-mouse Allergin-1
C57BL/6 mouse peritoneal cavity cells were stained with c-Kit PerCP, FcεR1α APC and Allergin-1 (clone TX83) PE (top), or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the c-Kit+ population.
