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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-MKK6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
MKK6 (Mitogen-activated protein kinase 6, MAPK/ERK kinase 6) is a 37 kD serine/threonine kinase family, MAP kinase subfamily. Two isoforms of MKK6 have been reported; MKK6 (only heart), MKK6B muscle, heart, liver, and pancreas. MKK6 phosphorylates and activates MAP kinase p38 and stress-activated kinase JNK. MKK6 is activated by UV, anisomycin, and osomotic shock. MKK6 is phosphorylated (Ser 207/Thr 211). MKK6 has been shown to interact with ASK-1 kinase. The Poly6047 antibody recognizes the N-terminal region of both human and mouse MKK6 and has been shown to be useful for Western blotting.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-MKK6
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-MKK6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
