This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation:
The antibody was purified by protein G affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
Upon receipt, store undiluted at 4° C.
Application:
WB - Quality Tested IP, IHC(Frozen)*, ICC*, ELISA * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Mouse brain tissue extract was resolved by electrophoresis, transferred nitrocellulose, and probed with monoclonal anti-MAP2ab (clone MT-08) antibody. Proteins were visualized using HRP goat anti-mouse IgG and a chemiluminescence detection system.
Description:
Microtubule associated protein 2 also know as MAP2, has three isoforms that include two high molecular weight forms MAP2A and MAP2B (~280KD), and a low molecular weight form MAB2C (~70KD). MAP2 belongs to the microtubule-associated protein family and expressed in the nervous system. The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. MAP2 has been shown to interact with Fyn, Grb2, PRKACA, and CPEB1. The MT-08 antibody has been shown to be useful for Western blotting, Immunoprecipitation, Immunohistochemistry (frozen sections), Immunocytochemistry, and ELISA of the human, mouse and porcine MAP2A and MAP2B isoforms.
Other Names:
Microtubule associated protein 2, MAP2
Structure:
MAP2 belongs to the microtubule-associated protein family. MAP2 has three isoforms that include two high molecular weight forms MAP2A and MAP2B (~280KD), and a low molecular weight form MAB2C (~70KD).
Distribution:
Cytoplasm protein, expressed in the nervous system.
Function:
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Interaction:
Fyn, Grb2, PRKACA, and CPEB1
Antigen References:
1. Garner, C. C., et al., 1988. Nature 336: 674. 2. Kindler, S., et al., 1994. Molec. Brain Res. 26: 218. 3. Chang, L., et al., 2003. Dev. Cell 4:521.
