Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Mouse brain tissue extract was resolved by electrophoresis, transferred nitrocellulose, and probed with monoclonal anti-MAP2ab (clone MT-08) antibody. Proteins were visualized using HRP goat anti-mouse IgG and a chemiluminescence detection system.
Description:
Microtubule associated protein 2 also know as MAP2, has three isoforms that include two high molecular weight forms MAP2A and MAP2B (~280KD), and a low molecular weight form MAB2C (~70KD). MAP2 belongs to the microtubule-associated protein family and expressed in the nervous system. The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. MAP2 has been shown to interact with Fyn, Grb2, PRKACA, and CPEB1. The MT-08 antibody has been shown to be useful for Western blotting, Immunoprecipitation, Immunohistochemistry (frozen sections), Immunocytochemistry, and ELISA of the human, mouse and porcine MAP2A and MAP2B isoforms.
Other Names:
Microtubule associated protein 2, MAP2
Structure:
MAP2 belongs to the microtubule-associated protein family. MAP2 has three isoforms that include two high molecular weight forms MAP2A and MAP2B (~280KD), and a low molecular weight form MAB2C (~70KD).
Distribution:
Cytoplasm protein, expressed in the nervous system.
Function:
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Interaction:
Fyn, Grb2, PRKACA, and CPEB1
Antigen References:
1. Garner, C. C., et al., 1988. Nature 336: 674. 2. Kindler, S., et al., 1994. Molec. Brain Res. 26: 218. 3. Chang, L., et al., 2003. Dev. Cell 4:521.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
