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Purified anti-MCM6 Antibody
Purified anti-MCM6 Antibody
602602 200 µl (20 Western blots) $225.00     
Product Details
Clone: Poly6026
Isotype: Rabbit IgG
Reactivity: Mouse, Human
Immunogen: Peptide-KLH (KYLQLAEELIRPERNT)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling:

Upon receipt, store frozen at -20° C.
Application:

WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
References: 		1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
2. WuZQ,et al. 2008. Proc Natl Acad Sci USA. 105:1919.PubMed
Hela (left lane) and NIH-3T3
Hela (left lane) and NIH-3T3 (right lane) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM6 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.


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Description: The MCM6 protein (minichromosome maintenance protein 6) is a nuclear 115 kD DNA-dependent ATPase. The MCM6 protein is a component of the DNA pre-replication complex that acts to allow DNA to undergo single round of replication per cell cycle. MCM6 together with MCM4 and MCM7 have DNA helicase activity. MCM6 has been reported to interact with the MCM4 and MCM7 (to form a 600 kD complex with helicase activity). The Poly6026 antibody recognizes both human and mouse MCM6 and has been shown to be useful for Western blotting.
Other Names: Minichromosome maintenance protein 6
Structure: DNA-dependent ATPase; 115 kD
Distribution: Nuclear
Function: Component of DNA pre-replication complex
Interaction: MCM4, MCM7 (600 kD complex with helicase activity)
Antigen
References: 		1. Hu B, et al. 1993. Nucleic Acids Res. 21:5289.
2. Ishimi Y. 1997. J. Biol. Chem. 272:24508.
GeneID: 4175
Latest Publications: View the latest MCM6 articles on HighwirePress.com
UniProt: View information about MCM6 on UniProt.org
Keywords: Purified anti-MCM6, Poly6026, Purified, Minichromosome maintenance protein 6 , Mouse, Human, Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-MCM6
    Hela (left lane) and NIH-3T3

    Hela (left lane) and NIH-3T3 (right lane) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM6 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

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