This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Tan, B. C-M., et al., 2006. EMBO doi: 10.1038/sj.emboj.7601271
Jurkat cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM5 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Description:
The MCM5 protein (minichromosome maintenance protein 5) is a nuclear 82 kD DNA-dependent ATPase. The MCM5 protein is a component of the DNA pre-replication complex that acts to allow DNA to undergo single round of replication per cell cycle. MCM5 is upregulated in the G0/G1 phase of the cell cycle. MCM5 has been reported to interact with the MCM3. The Poly6025 antibody recognizes both human and mouse MCM5 and has been shown to be useful for Western blotting.
Other Names:
Minichromosome maintenance protein 5
Structure:
DNA-dependent ATPase, approximately 82 kD
Distribution:
Nuclear
Function:
Component of DNA pre-replication complex
Regulation:
Upregulated in G0 to G1/S cell cycle phase
Interaction:
MCM3
Antigen References:
1. Hu, B., et al., 1993. Nuleic Acids Res. 21:5289. 2. Paul, R., et al., 1996. Cytogenet. Cell Genet. 73:317. 3. Burkhart, R., et al., 1995. Eur. J. Biochem. 228:431.
