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Purified anti-MCM5 Antibody
Purified anti-MCM5 Antibody
602502 200 µl (20 Western blots) £144     
Product Details
Clone: Poly6025
Isotype: Rabbit IgG
Reactivity: Mouse, Human
Immunogen: Peptide
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
References:

1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
Jurkat cell nuclear extract was
Jurkat cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM5 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.


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Description:

The MCM5 protein (minichromosome maintenance protein 5) is a nuclear 82 kD DNA-dependent ATPase. The MCM5 protein is a component of the DNA pre-replication complex that acts to allow DNA to undergo single round of replication per cell cycle. MCM5 is upregulated in the G0/G1 phase of the cell cycle. MCM5 has been reported to interact with the MCM3. The Poly6025 antibody recognizes both human and mouse MCM5 and has been shown to be useful for Western blotting.
Other Names: Minichromosome maintenance protein 5
Structure: DNA-dependent ATPase, approximately 82 kD
Distribution: Nuclear
Function: Component of DNA pre-replication complex
Regulation: Upregulated in G0 to G1/S cell cycle phase
Interaction: MCM3
Antigen
References:

1. Hu B, et al. 1993. Nuleic Acids Res. 21:5289.
2. Paul R, et al. 1996. Cytogenet. Cell Genet. 73:317.
3. Burkhart R, et al. 1995. Eur. J. Biochem. 228:431.
GeneID: 4174
Latest Publications: View the latest MCM5 articles on HighwirePress.com
UniProt: View information about MCM5 on UniProt.org
Keywords: Purified anti-MCM5, Poly6025, Purified, Minichromosome maintenance protein 5 , Mouse, Human, Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-MCM5
    Jurkat cell nuclear extract was

    Jurkat cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM5 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

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