Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Tan, B.C., et al. 2006. EMBOJ. 25:3975. (ChIP)
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM4 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Description:
The MCM4 protein (minichromosome maintenance protein 4) is a nuclear 100 kD DNA-dependent ATPase. The MCM4 protein is a component of the DNA pre-replication complex that acts to allow DNA to undergo single round of replication per cell cycle. MCM4 together with MCM6 and MCM7 have DNA helicase activity. MCM4 can be modified by phosphorylation by CDK2. Phosphorylation of MCM4 inhibits DNA replication. MCM4 has been reported to interact with the MCM6 and MCM7 (to form a 600 kD complex with helicase activity) and CDK2. The Poly6024 antibody recognizes human MCM4 and has been shown to be useful for Western blotting.
Other Names:
Minichromosome maintenance protein 4
Structure:
DNA-dependent ATPase; 100 kD
Distribution:
Nuclear
Function:
Component of DNA pre-replication complex, DNA helicase activity with MCM6, MCM7
Regulation:
Phosphorylated by CDK2, inhibits DNA replication
Modification:
Phosphorylation
Interaction:
MCM6, MCM7 (600 kD complex with helicase activity), CDK2
Antigen References:
1. Hu, B., et al., 1993. Nucleic Acids Res. 21:5289. 2. Ishimi, Y. 1997. J. Biol. Chem. 272:24508. 3. Ishimi, Y., et al., 2003. J. Biol. Chem. 278:24644.
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