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Purified anti-MCM3 Antibody
Purified anti-MCM3 Antibody
602302 200 µl (20 Western blots) $225.00     
Product Details
Clone: Poly6023
Isotype: Rabbit IgG
Reactivity: Mouse, Human
Immunogen: Peptide
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
References:

1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271.
2. Yim H, et al. 2009. Mol Cell Biol. 29:2609. PubMed
Hela cell nuclear extract was
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.


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Description:

The MCM3 protein (minichromosome maintenance protein 3) is a 110 kD DNA-dependent ATPase containing a nuclear localization sequence. The MCM3 protein is a component of the DNA pre-replication complex that acts to allow DNA to undergo single round of replication per cell cycle. MCM3 is constitutively expressed and can be modified by acetylation. Acetylation of MCM3 inhibits the initiation of DNA replication. MCM3 has been reported to interact with the MCM5 (CDC46) and MCM3AP proteins. The Poly6023 antibody recognizes both human and mouse MCM3 and has been shown to be useful for Western blotting.
Other Names: Minichromosome maintenance protein 3
Structure: DNA-dependent ATPase, nuclear localization sequence; 110 kD
Distribution: Nuclear
Function: Component of DNA pre-replication complex. Allows DNA to undergo single round of replication per cell cycle
Regulation: Constitutively expressed, acetylation inhibits the initiation of DNA replication
Modification: Acetylation
Interaction: MCM5 (CDC46), MCM3AP
Antigen
References:

1. Hu B, et al. 1993. Nucleic Acids Res. 21:5289.
2. Ishimi Y. 1997. J. Biol. Chem. 272:24508.
3. Ishimi Y, et al. 2003. J. Biol. Chem. 278:24644.
GeneID: 4172
Latest Publications: View the latest MCM3 articles on HighwirePress.com
UniProt: View information about MCM3 on UniProt.org
Keywords: Purified anti-MCM3, Poly6023, Purified, Minichromosome maintenance protein 3, Mouse, Human, Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-MCM3
    Hela cell nuclear extract was

    Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

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