Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. ZhaoY,et al.2007.Mol Cell Biol.27:4328.PubMed 2. Perperzak V, et al. 2010. J. Immunol. 185:6670. PubMed 3. Hu L, et al. 2011 J. Virol. PubMed 4. Coloff JL, et al. 2011. J. Biol Chem, 18:286. PubMed
A20 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Mcl-1 polyclonal antibody. Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Mcl-1 (myeloid cell leukemia-1) is a 37 kD Bcl-2 related protein found in mitochondrial and non-mitochondrial membranes that dimerizes with Bax. Mcl-1 has been shown to act as a survival factor for hematopoietic cells and contribute to the STAT-dependent protection of neutrophils against apoptosis. Mcl-1 is increased by PMA, GM-CSF, M-CSF, IL-1β, IL-3, and IL-6 in hematopoietic cells. This protein is decreased following growth factor withdrawal. Mcl-1 is modified by JNK phosphorylation. The Poly6136 antibody has been shown to recognize mouse Mcl-1 and has been shown to be useful for Western blotting.
Other Names:
Myeloid cell leukemia-1
Structure:
Bcl-2 related protein; 37kD
Distribution:
Mitochondrial, nonmitochondrial membranes
Function:
Survival factor for hematopoietic cells, contributes to STAT-dependent protection of neutrophil apoptosis
Regulation:
Increased by PMA, GM-CSF, M-CSF, IL-1β, IL-3, and IL-6 in hematopoietic cells. Decreased after growth factor withdrawal
Modification:
Phosphorylation by JNK
Interaction:
Dimerizes with Bax
Antigen References:
1. Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA 90:3516. 2. Zhou P, et al. 1997. Blood 89:630. 3. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883. 4. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486. 5. Moulding D, et al. 1998. Blood 92:2495. 6. Inoshita S, et al. 2002. J. Biol. Chem. 277:43740.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Mcl-1
A20 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Mcl-1 polyclonal antibody. Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
