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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.8-1.5 µg antibody per ml dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The optimal concentration should be determined by titration for each individual assay of interest.
Hela total cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MAPKAP1 polyclonal Poly6488. Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
SIN1 encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Recent studies have shown that it is identical to MIP1 for MEKK2 interacting protein 1. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. It inhibits HRAS and KRAS signaling and enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
Other Names:
Mitogen-activated protein kinase associated protein 1; Target of rapamycin complex 2 subunit MAPKAP1; SAPK-interacting protein 1; Sin1; MIP1
Structure:
522 amino acids; 59123 Da. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.
This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. It is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals.
Interaction:
All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2, MAPK8, GTP-bound HRAS and KRAS.
Antigen References:
1. Wullschleger S, et al. 2006. Cell 124:471. 2. Jacinto E, et al. 2006. Cell 127:125. 3. Schroder W, et al. 2005. Cell. Signal. 17:761.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-MAPKAP1
Hela total cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MAPKAP1 polyclonal Poly6488. Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
