This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MOLT-4 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MAD2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
MAD2 (also known as mitotic arrest deficient and mitotic spindle assembly checkpoint protein MAD2A) is a 24 kD nuclear protein containing a HORMA domain. MAD2 acts as a mitotic checkpoint, monitoring kinetochore-spindle attachment, and delaying anaphase if process incomplete. MAD2 inhibits APC activity by sequestering Cdc20. MAD2 interacts with several binding partners; affinity for binding partners changes in a cell cycle-dependent manner. MAD2 has been reported to interact with Cdc20, and ADAM17 (TACE). The Poly6021 antibody recognizes the N-terminal region of human MAD2 and has been shown to be useful for Western blotting.
Other Names:
Mitotic arrest deficient, Mitotic spindle assembly checkpoint protein MAD2A
Structure:
Mad2, HORMA domain; 24 kD
Distribution:
Nuclear
Function:
Mitotic checkpoint, monitors kinetochore-spindle attachment, delays anaphase if process incomplete. Inhibits APC activity by sequestering Cdc20
Regulation:
Affinity for binding partners changes in a cell cycle-dependent manner
Interaction:
Cdc20, ADAM17 (TACE)
Antigen References:
1. Fang, G., et al., 1998. Genes Dev. 12:1871. 2. Nelson, K., et al., 1999. Biochem. J. 343:673. 3. Luo, X., et al., 2000. Nat. Struct. Biol. 7:224. 4. Shannon K., et al., 2002. Mol. Biol. Cell. 13:3706.
