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Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Draberova L, et al. 1996. Immunology 87:141. 2. Amoui M, et al. 1997 Eur. J. Immunol. 27:321. 3. Kovarova M, et al. 2001. Mol. Cell Biol. 21:8318.
Extracts from peripheral blood mononuclear cells were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Lyn (clone LYN-01) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Lyn is a tyrosine protein kinase and a member of the src subfamily. Two isoforms of Lyn have been reported: Lyn A, 53 kD and Lyn B, 56 kD. Lyn is predominantly expressed in macrophages, B cells, and platelets. Lyn exhibits both stimulatory activity (mast cells) and inhibitory activity (B cells), and has been shown to increase p53 levels and inhibit nuclear export. Recent reports have suggested that Lyn may participate in the induction of apoptosis by genotoxic agents. Lyn has been shown to be activated in late G1 by stem cell factor and is increased in myeloid leukemias. Lyn interacts with the high affinity IgE receptor, actin cytoskeleton, γ-tubulin, and p53. The LYN-01 monoclonal antibody recognizes the human, mouse, and rat Lyn and has been shown to be useful for Western blotting, immunoprecipitation, and immunocytochemistry.
Other Names:
Tyrosine protein kinase Lyn
Structure:
Tyrosine family of protein kinases, src subfamily. Two isoforms Lyn A, Lyn B, 53 kD, 56 kD
Distribution:
Predominantly expressed in macrophages, B cells, platelets
Function:
Both stimulatory (mast cells) and inhibitory (B cells) function in hematopoietic cells. Increases p53 levels and inhibits nuclear export, participates in induction of apoptosis by genotoxic agents
Regulation:
Activated in late G1 in stem cell factor induced cell cycle progression, increased in myeloid leukemias
Modification:
Phosphorylation, Ubiquitination
Interaction:
High affinity IgE receptor, actin cytoskeleton, γ-tubulin, p53
Antigen References:
1. Yamanashi Y, et al. 1987. Mol. Cell. Biol. 7:237. 2. Rider L, et al. 1994. Gene 138:219.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Lyn
Extracts from peripheral blood mononuclear cells were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Lyn (clone LYN-01) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
