This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
WB-Quality Tested IP, FC, ICC* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Romagnoli P., et al., 2001. Int. Immunol. 13: 305.
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Lck (clone LCK-01) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Lck is a 56 kD tyrosine protein kinase and a member of the src subfamily that contains both an SH2 and SH3 domain. Alternatively spliced isoforms of Lck have been reported. Lck is a cytoplasmic protein bound to CD4 and CD8 in T lymphocytes that participates in antigen-induced T cell activation through phosphorylation of the T cell receptor zeta chain. Lck is activated upon T cell receptor engagement and is involved in the onset of cell cycle progression induced by interleukin-2. Phosphorylation by Csk downregulates the activity of Lck. In addition to CD4 and CD8, Lck has been reported to interact with PI3K through its SH3 domain and tyrosine phosphorylated KHDRBS1/p70 through its SH2 domain. In addition, Lck has been shown to associate with the SAP transmembrane tyrosine phosphatase. The Lck-01 monoclonal antibody recognizes human Lck and has been shown to be useful for Western blotting, immunoprecipitation, immunocytochemistry, and flow cytometry.
Other Names:
Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase< p56-LCK, LSK, T cell-specific protein-tyrosine kinase>
Structure:
Belongs to the tyrosine family of kinases, src subfamily. Contains one SH2 domain, 1 SH3 domain. Approximately 56 kD, alternatively spliced isoforms have been reported
Distribution:
Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes
Function:
Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression
Regulation:
Activated upon T cell receptor engagement. Tyrosine phosphorylation by p50 Csk downregulates catalytic activity
Modification:
Phosphorylation
Interaction:
Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase
Antigen References:
1. Koga, Y., et al., 1986. Eur. J. Immunol. 16:1643. 2. Vogel, L. B., et al., 1995. Biochem. Biophys. Acta 1264:168. 3. Vogel, L. B., et al., 1993. Mol. Cell. Biol. 13:7408. 4. Bergaman, M., et al., 1992. EMBO J. 11:2919.
