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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Dorn T, et al. 2007. Blood 109:2346. 2. Sloan D, et al. 2006. J. Immunol. 176:1825
Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12.
LAT is a transmembrane molecule that mediates signaling following the engagement of a number of cell surface receptors including TCR during mature T cell activation, pre-TCR during T cell development, Fc RIII on NK cells, and FcεRI on mast cells.LAT is multiply phosphorylated on tyrosines by ZAP-70 and Syk, which leads to downstream effects that include release of intracellular calcium, PKC activation, MAPK activation and cytoskeletal reorganization.Palmitoylation of LAT is required for its localization to lipid rafts and efficient phosphorylation.
Other Names:
Linker for activation of T-cells family member 1, pp36, p36-38 < 36 kD phospho-tyrosine adapter protein, pp36, p36-38>
Structure:
Type III transmembrane protein, MW of 28 kD but expected at 36-38 kD due to post-translational modifications including palmitoylation
Distribution:
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
Function:
LAT is required for signaling through a number of cell surface receptors including TCR, FcγRIII, and FcεRI.
Regulation:
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation
Interaction:
Interacts directly with GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, LCP2, SHB, SKAP2, MIST,and FcγR1A
Antigen References:
1. Zhang W et al. 1998. Cell 92:83. 2. Weber JR. et al. 1998. J. Exp. Med. 187:1157
Purified anti-LAT, 11B.12, Purified, Linker for activation of T-cells family member 1, pp36, p36-38 < 36 kD phospho-tyrosine adapter protein, pp36, p36-38>, Human, Cross-Reactivity: Mouse, Immunology, Antibodies
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Purified anti-LAT
Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12.
