GST fusion protein corresponding to residues 31-233 of human LAT, cytoplasmic domain.
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation:
The antibody was purified by protein G affinity chromatography
Concentration:
0.5 mg/ml
Storage & Handling:
Upon receipt, store at 4° C
Application:
WB - Quality Tested IP - This application was tested during development.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Dorn, T. et al. 2007. Blood. 109:2346. 2. Sloan, D. et al. 2006. J. Immunol. 176:1825
Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12.
Description:
LAT is a transmembrane molecule that mediates signaling following the engagement of a number of cell surface receptors including TCR during mature T cell activation, pre-TCR during T cell development, FcgRIII on NK cells, and FcεRI on mast cells.LAT is multiply phosphorylated on tyrosines by ZAP-70 and Syk, which leads to downstream effects that include release of intracellular calcium, PKC activation, MAPK activation and cytoskeletal reorganization.Palmitoylation of LAT is required for its localization to lipid rafts and efficient phosphorylation.
Other Names:
Linker for activation of T-cells family member 1, pp36, p36-38 < 36 kDa phospho-tyrosine adapter protein, pp36, p36-38>
Structure:
Type III transmembrane protein, MW of 28 kD but expected at 36-38 kD due to post-translational modifications including palmitoylation
Distribution:
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
Function:
LAT is required for signaling through a number of cell surface receptors including TCR, FcγRIII, and FcεRI.
Regulation:
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation
Interaction:
Interacts directly with GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, LCP2, SHB, SKAP2, MIST,and FcγR1A
Antigen References:
1. Zhang, W. et al. 1998. Cell 92:83. 2. Weber, J.R. et al. 1998. J. Exp. Med. 187:1157
