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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Zhang Y, et al. 2007. Mol Cancer Res. 5:909. PubMed
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-lamin A. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Lamin A is a member of the intermediate filament family that contains a farnesyl binding domain and forms dimers. Three isoforms of Lamin A have been reported designated A, AD10, C, with molecular weights of approximately 70 kD, 66 kD, and 61 kD, respectively. Lamin A is localized to the nucleoplasmic side of inner nuclear membrane. Lamin A is thought to function as a fibrous component of the nuclear lamina, providing a framework for the nuclear envelope, and possibly interacting with chromatin. Expression of lamin A is strictly under cell cycle control as seen by disintegration/formation of nuclear envelope in prophase/telophase. Lamin A is modified by phosphorylation, methylation, and farnesylation; phosphorylation regulates disassembly. Lamin A forms a homodimer with Lamin C and has also been shown to interact with the LAPs 1A-1C, emerin, Narf, hsMOK2, PKC, SREBP1a, and SREBP 1c. The Poly6135 antibody recognizes the C-terminal region of human and mouse Lamin A and has been shown to be useful for Western blotting and immunofluorescence staining.
Intermediate filament family, farnesyl binding domain, dimer. Isoforms A, AD10, C, approximately 70 kD, 66 kD, and 61 kD, respectively
Distribution:
Nucleoplasmic side of inner nuclear membrane
Function:
Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
Regulation:
Strictly under cell cycle control as seen by disintegration/formation of nuclear envelope in prophase/telophase. Phosphorylation regulates disassembly
Modification:
Phosphorylation, Methylation, Farnesylation
Interaction:
Homodimeric with Lamin C, interacts with LAPs 1A-1C, emerin, Narf, hsMOK2, PKC, SREBP1a, SREBP 1c
Antigen References:
1. Machiels B, et al. 1996. J. Biol. Chem. 271:9249. 2. Barton R, et al. 1999. J. Biol. Chem. 274:30008. 3. Gruenbaum Y, et al. 2000. J. Struct. Biol. 129:313. 4. Stierle V, et al. 2003. Biochemistry 42:4819.
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Purified anti-Lamin A
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-lamin A. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
