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Purified anti-Ku80 Antibody
Purified anti-Ku80 Antibody
608302 100 µg $185.00     
Product Details
Clone: F3
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: Recombinant
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was protein G-purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store at 4°C.
Application: WB, IF
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 μg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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 Hela cell lysate was resolved
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Ku80 antibody. Proteins were visualized using an anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.


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Description:

Ku80 is an 80 kD nuclear ATP-dependent DNA helicase. Ku80 is involved in dsDNA break repair, VDJ recombination, and chromosome translocation. This protein binds fork-like ends of dsDNA in cell-cycle dependent manner. Ku80 can be modified by phosphorylation and has been shown to interact with Ku70 and DNA-PKcs. The F3 monoclonal antibody recognizes human Ku80 and has been shown to be useful for Western blotting and immunofluorescence staining.
Other Names: ATP-dependent DNA helicase II, Lupus Ku autoantigen protein
Structure: ssDNA-dependent ATP-dependent helicase; 80 kD
Distribution: Nuclear
Function: dsDNA break repair, VDJ recombination, chromosome translocation
Regulation: Binds fork-like ends of dsDNA in cell-cycle dependent manner
Modification: Phosphorylation
Interaction: Ku70, DNA-PKcs
Antigen
References: 		1. Yaneva M, et al. 1989. J. Biol. Chem. 264:13407.
2. Stuiver M, et al. 1990. J Exp Med. 172:1049.
3. Tuteja N, et al. 1994. EMBO J. 13:4991.
4. Song J, et al. 2003. J. Biol. Chem. 278:36676.
GeneID: 22596
Latest Publications: View the latest Ku80 articles on HighwirePress.com
UniProt: View information about Ku80 on UniProt.org
Keywords: Purified anti-Ku80, F3, Purified, ATP-dependent DNA helicase II, Lupus Ku autoantigen protein
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-Ku80
    Hela cell lysate was resolved

    Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Ku80 antibody. Proteins were visualized using an anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

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