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Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 25 µl per 5 ml antibody dilution buffer for each mini-gel.It is recommended that the reagent be titrated for optimal performance for each application.
COA:
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified polyclonal anti-JNK1 antibody (clone Poly6331). Two isoforms around 40-45 kD were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
The MAPK family includes several subgroups encompassing the extracellular signal-regulated kinase (ERK) family, the p38 Map kinase family, and c-Jun NH2-terminal kinase (JNK) family. JNK kinase group includes 3 members, JNK1, JNK2, and JNK3. JNK kinases are phosphorylated in response to various activations by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulate AP-1 transcriptional activity. JNK kinases are characterized by requirement for dual phosphorylation at a T-P-Y motif for enzymatic activation. The Poly6331 antibody has been shown to be useful for western blotting, immunoprecipitation, and immunohistochemistry of human, mouse, and rat JNK1 p46 and JNK1 p54 protein.
Other Names:
Mitogen-activated protein kinase 8, MAPK8, SAPK1
Structure:
Mitogen-activated protein kinase family member, dual specificity kinase, 40-45 kD
Distribution:
Ubiquitous cytoplasmic protein, translocates to nucleus upon activation.
Interaction:
c-Jun, Bcl2, MAP2K4, MAP2K7, ATF2
Bioactivity/Activities:
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcriptional factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity.
Antigen References:
1. Derijard B, et al. 1994. Cell 76:1025 2. Tournier C, et al. 2000. Science 288:870 3. Dong C, et al. 2000. Nature 405:91
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Purified anti-JNK1
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified polyclonal anti-JNK1 antibody (clone Poly6331). Two isoforms around 40-45 kD were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
