Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
293T cell extracts from either UV treated or untreated control were resolved by electrophoresis, transferred to nitrocellulose, and probed with Purified anti-JNK Phospho (Thr183/Tyr185) Antibody (Poly6513). Proteins were visualized using anti-rabbit IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, lystates from untreated Hela cells; Lane 2, lysates from Hela cells treated with UV for 3 minutes.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is activated by various cell stimuli and targets specific transcription factors, thus mediating immediate-early gene expression in response to cell stimuli. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase plays a key role in T cell proliferation, apoptosis, and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Other Names:
Stress-activated protein kinase 1, Stress-activated protein kinase JNK1, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2
Distribution:
Cytoplasm. Translocates to nucleus in response to tyrosine phosphorylation.
Function:
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. It is important for T cell activation.
Interaction:
Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 andSPAG9/MAPK8IP4/JIP-4. Interacts with TP53 and WWOX and JAMP.
Antigen References:
1. Chen YR, et al. 1996. J. Biol. Chem. 271:631. 2. Su B, et al. 1994. Cell 77:727. 3. Brandt B, et al. 2010. Cell Death Dis. 1:e23. 4. Whitmarsh AJ, et al. 1998. Trends Biochem Sci. 23:481.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-JNK Phospho (Thr183/Tyr185)
293T cell extracts from either UV treated or untreated control were resolved by electrophoresis, transferred to nitrocellulose, and probed with Purified anti-JNK Phospho (Thr183/Tyr185) Antibody (Poly6513). Proteins were visualized using anti-rabbit IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, lystates from untreated Hela cells; Lane 2, lysates from Hela cells treated with UV for 3 minutes.
