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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Foster ML,et al.2006. J. Cell Biol.19:173.PubMed
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-IκBα polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Formalin-fixed paraffin-embedded human liver tissue was stained with Poly6249 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
IκB-α is an inhibitory protein that binds the NFκB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing nuclear translocation of NFκB. The NFκB complex binds DNA to activate gene expression of a number of a variety of proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis. Poly6249 recognizes the C-terminal region of human, mouse, rat IKB-α and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
Other Names:
Nuclear factor of kappa light chain enhancer in B cells inhibitor alpha, IKBA
Structure:
Inhibitory protein for NFκB complex, contains multiple ankyrin repeats, approximately 39 kD.
Distribution:
Cytoplasm
Function:
Regulates transcriptional activity of NFκB by binding complex and trapping in the cytoplasm. Serine phosphorylation of IκB proteins targets them for destruction via the ubiquitination pathway and allows the NFκB complex to translocate to
Modification:
Phosphorylation, ubiquitination, sumoylation
Interaction:
NFkB1, SUMO4, p53, ribosomal S6 kinase 1, and many others
Antigen References:
1. Baeuerle PA. 1998. Cell 95:729. 2. Hoffmann A et al. 2002. Science 298:1241. 3. Ito CY. et al. 1995. Genomics 29:490.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-IκB-α
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-IκBα polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Formalin-fixed paraffin-embedded human liver tissue was stained with Poly6249 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
