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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Immunofluorescence cell staining1, immunoprecipitation1, Western blotting1-2, ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
Application References:
1. Andrews PW, et al. 1984. Hybridoma. 3:347. 2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
HUES-9 ES cell colony was stained with Tra-1-81 antibody at 1:400 and then secondarily stained with Alexa Fluor® 546 goat anti-mouse IgM at 1:250 (red). Nuclei were counterstained with DAPI (blue). Image was provided by Eric O'Connor and Karl Marquez, Sanford Consortium for Regenerative Medicine.
Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE
The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.
Other Names:
Podocalyxin
Structure:
Mucin-like protein, CD34 family member, 200-240 kD
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human TRA-1-81
HUES-9 ES cell colony was stained with Tra-1-81 antibody at 1:400 and then secondarily stained with Alexa Fluor® 546 goat anti-mouse IgM at 1:250 (red). Nuclei were counterstained with DAPI (blue). Image was provided by Eric O'Connor and Karl Marquez, Sanford Consortium for Regenerative Medicine.
Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE
Biotin anti-human TRA-1-81
Human teratocarcinoma cell line, NCCIT, stained with biotinylated TRA-1-81, followed by Sav-PE
