Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 309709).
Application References:
1. Flo, T., et al., 2000. J. Immunol. 164:2064. 2. Faure, E., et al., 2001. J. Immunol. 166:2018. 3. Sugawara, S., et al., 2001. Infect. Immun. 69:4951. 4. Droemann, D., et al., 2003. Histochem. Cell Biol. 119:103. 5. Chavakis, E., et al., 2007. Circ. Res. 100:204.PubMed 6. Fiala, M., et al., 2007. Proc. Natl. Acad. Sci. USA 10.1073/pnas.0701267104. 7. Goo, S.Y., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed 8. Weiss, DJ.,et al. 2008.J Leukoc Biol. 83:48. PubMed
Human peripheral blood monocytes stained
with TL2.1 FITC
Description:
Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.
Other Names:
Toll like receptor 2, TIL4, CD282
Structure:
IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Distribution:
Monocytes, granulocytes, endothelial and epithelial cells
Function:
Activates innate host defense against bacterial pathogens
1. Lien, E., et al., 1999. J. Biol. Chem. 274:33419. 2. Lien, E. et al., 2001. J. Biol. Chem. 276:1873. 3. Sabroe, I., et al., 2002. J. Immunol. 168:4701
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