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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Human peripheral blood granulocytes were stained with purified TLT-2 (MIH60) (filled histogram) or mouse IgG2b, κ isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
Trem-like transcript 2 protein (TLT2), also known as TREML2, is a 33 kD (unglycosylated) type I transmembrane protein with a single IgV domain contains two potential N-glycosylation sites, a serine/threonine-rich region with numerous predicted O-linked glycosylation sites, a single membrane-spanning region, and a short cytoplasmic tail. TLT2 is expressed on B lineage cells, macrophages, neutrophils in bone marrow and periphery, and the expression level can be upregulated in response to inflammation. As a receptor, TLT2 likely plays a role in innate immunity. Recent studies have demonstrated that ligation of TLT2 inhibits the oxidative burst in neutrophils upon fMLP stimulation, indicating that TLT2 may function as an inhibitory receptor that attenuates activation of neutrophils, and possibly macrophages. Recently TLT-2 protein has been found on CD8+ T cells and is induced on activated CD4+ T cells.
Other Names:
TREML2
Structure:
Type I transmembrane protein with IgV type domain and one potential site of N linked glycosylation at extracellular domain
Distribution:
B cell, macrophages, neutrophils
Function:
Inhibitory receptor
Interaction:
B7H3
Antigen References:
1. Ford JW, et al. 2009. Curr. Opin. Immunol. 21:38. 2. Hashiguchi M, et al. 2008. P. Natl. Acad. Sci. USA 105:10495. 3. King RG, et al. 2006. J Immunol. 176:6012. 4. Leitner J, et al. 2009. Eur. J. Immunol. 39:1754. 5. Park-Min KH, et al. 2009. J. Immunol. 183:2444. 6. Hashiguchi M, et al. 2008. P. Natl. Acad. Sci. USA 105:10495.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human TLT-2
Human peripheral blood granulocytes were stained with purified TLT-2 (MIH60) (filled histogram) or mouse IgG2b, κ isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
PE anti-human TLT-2
Human peripheral blood granulocytes were stained with TLT2 (clone MIH60) PE (filled histogram) or mouse IgG2b PE isotype control (open histogram).
Human peripheral blood lymphocytes were stained with CD19 APC and TLT2 (clone MIH60) PE (middle) or mouse IgG2b isotype control (bottom).
