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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This clone has been published as 1H9.
Additional reported applications (for the relevant formats) include: Western blot1, immunohistochemical staining with paraffin-embedded tissues section1,2.
Application References:
1. Robinson MJ, et al. 2002. J. Biol. Chem. 277:3658. (WB, IHC) 2. Croce K, et al. 2009. Circulation 120:427. (IHC)
Human peripheral blood granulocytes surface stained with anti-human CD15 PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (bottom)
MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and recently has been considered an endogenous activator of Toll-like receptor 4. A recent report has identified S100A9 as a marker for CD14(+) HLA-DR(-/low) MDSCs.
Belongs to S100 family of protein containing 2-EF-hand calcium –binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8
Distribution:
MDSCs, Neutrophil, monocyte, and ileal tissue eosinophils
Function:
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction:
Calcium
Antigen References:
1. Loser K, et al. 2010. Nat. Med. 16:713. 2. Cheng P, et al. 2008. J. Exp. Med. 205:2235. 3. Leukert N, et al.2006. J. Mol. Biol. 359:961. 4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402. 5. Zhao F, et al. 2012. Immunology. 136:176.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human MRP-14 (S100A9)
Human peripheral blood granulocytes surface stained with anti-human CD15 PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (bottom)
FITC anti-human MRP-14 (S100A9)
Human peripheral blood granulocytes surface stained with anti-human CD15-PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (middle)
