Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
ELISA or ELISPOT Capture1-3,8: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202/501203) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1-3,6,7: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110).
Application References:
1. Abrams, J., et al., 1992. Immunol. Rev. 127: 5. 2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20. 3. Gaines Das, R., et al., 1993. J. Immunol. Meth. 160: 147. 4. Enriquez, J., et al., 2002. Adv. Perit Dial. 18: 177. 5. Andersson, U., et al., 1999. Detection and quantification of gene expression. New York: Springer-Verlag. 6. Zou, J-p., et al., 1999. J. Immunol. 162:4882. 7. Wyant, T. L., et al., 1999. Infect. Immun. 67:1338. 8. Lesmeister, M. J., et al., 2005. Reproductive Biology and Endocrinology 3:74. 9. Teresaka, Y., et al. 2010. Invest Opthalmol Vis Sci. 51:2441. PubMed.
Description:
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.
Other Names:
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Structure:
Neuropoietic cytokine family; 26 kD (Mammalian)
Regulation:
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Cellular Sources:
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cellular Targets:
T cells, B cells, hepatocytes, cholinergic neurons
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hirano, T. 1998. Intl. Rev. Immunol. 16:249. 3. Patterson, P. 1992. Curr. Opin. Neurobiol. 2:94. 4. Van Oers, M., et al., 1993. Ann. Hematology 66:219.
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