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Purified Anti-human IL-6 Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human IL-6 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human IL-6 Antibody
501101 50 µg $70.00     
501102 500 µg $200.00     
Clone: MQ2-13A5
Isotype: Rat IgG1, κ
Reactivity: Human
Immunogen: COS-7- expressed, recombinant human IL-6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application: ELISA Capture1-3 - Quality tested
IHC5*, WB * - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
ELISA or ELISPOT Capture1-3,8: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202/501203) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1-3,6,7: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110).
Application
References:
1. Abrams, J., et al., 1992. Immunol. Rev. 127: 5.
2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
3. Gaines Das, R., et al., 1993. J. Immunol. Meth. 160: 147.
4. Enriquez, J., et al., 2002. Adv. Perit Dial. 18: 177.
5. Andersson, U., et al., 1999. Detection and quantification of gene expression. New York: Springer-Verlag.
6. Zou, J-p., et al., 1999. J. Immunol. 162:4882.
7. Wyant, T. L., et al., 1999. Infect. Immun. 67:1338.
8. Lesmeister, M. J., et al., 2005. Reproductive Biology and Endocrinology 3:74.





Description: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.
Other Names: Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Structure: Neuropoietic cytokine family; 26 kD (Mammalian)
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Cellular Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cellular Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Bioactivity/Activities: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Antigen
References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano, T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson, P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers, M., et al., 1993. Ann. Hematology 66:219.
Biotin anti-human IL-6MQ2-39C3ELISA Detection,ELISPOT Detection
Avidin-HRPAvidinELISA,ELISPOT,WB
TMB Substrate Reagent SetELISA
ELISA Assay Diluent (5X)ELISA
Human IL-6 ELISA MAX™ StandardELISA
Human IL-6 ELISA MAX™ DeluxeELISA