Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
ELISA3 or ELISPOT4 Capture: The purified 8D4 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP4-25D2 antibody (Cat. No. 500804/500816) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500707).
Application References:
1. Bird, C., et al., 1991. Cytokine. 3: 562. 2. Prussin, C., et al., 1995. J. Immunol. Meth. 188: 117. 3. Hickie, I., et al., 2001. Twin Res. 4: 94. 4. Heeger, P., et al., 1999. J. Immunol. 163: 2267. 5. Jason, J., et al., 1999. Clin. Diagn. Lab Immunol. 6: 73. 6. Andersson, U., et al., 1999. Detection and quantification of gene expression. New York: Springer-Verlag. 7. Gong, G., et al. 2008. Blood. PubMed
Description:
IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. The 8D4-8 antibody can neutralize the bioactivity of natural or recombinant IL-4.
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Cellular Sources:
Mast cells, T cells, bone marrow stromal cells
Cellular Targets:
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors:
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Bioactivity/Activities:
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Boulay, J., et al., 1992. Curr. Opin. Immunol. 4:294. 3. Dullens, H., et al., 1991. In vivo 5:567. 4. Paul, W. 1991. Blood. 77:1859.
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