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Each lot of this antibody is quality control tested by Western blotting. For Western blotting applications, a concentration of 1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The optimal concentration should be determined by titration for each individual assay of interest.
500 ng (Lane 1), 200 ng (Lane 2), and 50 ng (Lane 3) of human IL-33 (Ser112-Thr270) recombinant proteins were separated by electrophoresis, transferred to nitrocellulose, and probed with monoclonal hIL-33 antibody (clone BL35172). Protein were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.
Other Names:
human Interleukin-33, DV27, C9ORF26, IL1F11 (Interleukin-1 Family Member 11), NFHEV (Nuclear factor from high endothelial venules)
Structure:
270 aa with predicted molecular weight of 30 kD
Distribution:
Full length IL-33 localizes to the nucleus in HUVECs and transfectants C-terminal fragment, corresponding mature IL-33 is secreted.
Function:
Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.
Interaction:
Binding to IL1RL1/ST2 and IL-1 Receptor Accessory Protein
Antigen References:
1. Lohning M, et al. 1998. P. Natl. Acad. Sci. USA 95:693. 2. Onda H, et al. 1999. J. Cerebr. Blood F. Met. 19:1279. 3. Schmitz J, et al. 2005. Immunity 23:479. 4. McLaren JE, et al. 2010. J. Immunol. 185:1222.
Purified anti-human IL-33, BL35172, Purified, human Interleukin-33, DV27, C9ORF26, IL1F11 (Interleukin-1 Family Member 11), NFHEV (Nuclear factor from high endothelial venules), Human; This antibody does not cross react with mouse IL-33., Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human IL-33
500 ng (Lane 1), 200 ng (Lane 2), and 50 ng (Lane 3) of human IL-33 (Ser112-Thr270) recombinant proteins were separated by electrophoresis, transferred to nitrocellulose, and probed with monoclonal hIL-33 antibody (clone BL35172). Protein were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
