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Purified Anti-human IL-10 Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human IL-10 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human IL-10 Antibody
506801 50 µg $70.00     
506802 500 µg $200.00     
Clone: JES3-19F1
Isotype: Rat IgG2a, κ
Reactivity: Human
Immunogen: COS-expressed recombinant human IL-10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application: ELISA Capture1-4 - Quality tested
IHC5,6*, WB * - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
ELISA or ELISPOT Capture1-4: The purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502/501503) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1,2: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. No. 506810).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.
Application
References:
1. Abrams, J., et al., 1992. Immunol. Rev. 127: 5.
2. Gotlieb, W., et al., 1992. Cytokine 4: 385.
3. Yssel, H., et al., 1992. J. Immunol. 149: 2378.
4. Burdin, N., et al., 1993. J. Exp. Med. 177: 295.
5. Andersson, U., et al., 1999. Detection and quantification of gene expression. New York: Springer-Verlag.
6. Andersson, J., et al., 1994. Immunology 83: 16.


Description: IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-19F1 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-19F1 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Other Names: Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
Structure: Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
Regulation: Production inhibited by IL-4, IL-10
Cellular Sources: Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cellular Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Bioactivity/Activities: Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Antigen
References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefyt, R., et al., 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al., 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.
Biotin anti-human IL-10JES3-12G8ELISA Detection,ELISPOT Detection
Avidin-HRPAvidinELISA,ELISPOT,WB
TMB Substrate Reagent SetELISA
ELISA Assay Diluent (5X)ELISA
Human IL-10 ELISA MAX™ StandardELISA
Human IL-10 ELISA MAX™ DeluxeELISA