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Purified anti-human IgG Fc Antibody
Cat. # Size Price  

409301 50 µg $65.00
    
409302 500 µg $125.00
    
Product Details

Clone: HP6017 (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control:Purified Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human, Cross-Reactivity: Goat, Rabbit, Sheep, Horse
Immunogen: Purified human immunoglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Clone HP6017 reacts with IgG (Fc fragment) of all subclasses. This antibody does not react with IgA, IgM, purified light chains, or the Fab fragment of IgG. Initial testing suggests that this antibody does not detect transmembrane IgG on B cells.
Application
References:
  Publication Library
 Image 1
Human DLL4 transfected CHO cells were incubated with (top) or without (bottom) recombinant human Notch 1-IgG Fc fusion protein and then stained with human IgG Fc (clone HP6017) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).

 Image 2


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Antigen Details

Description: IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.
Structure: Homodimer formed by the constant region of the IgG heavy chain
Ligand Receptor: CD16, CD32, CD64
Antigen
References:
1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.
GeneID: 3500
UniProt: View information about IgG Fc on UniProt.org
Keywords: Purified anti-human IgG Fc, HP6017, Purified, Human, Cross-Reactivity: Goat, Rabbit, Sheep, Horse, Flow Cytometry, Immunology, Antibodies
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Version: 2 Revision Date: 2014-08-08
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) biotin (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by Sav-PE.

  • APC anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).

  • Alexa Fluor® 647 anti-human IgG Fc
    Human DLL4-transfected CHO cells were

    Human DLL4-transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).

  • Purified anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with (top) or without (bottom) recombinant human Notch 1-IgG Fc fusion protein and then stained with human IgG Fc (clone HP6017) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).





  • PE anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with (top) or without (bottom) recombinant human Notch 1-IgG Fc fusion protein and then stained with human IgG Fc (clone HP6017) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).





  • FITC anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).

  • PerCP/Cy5.5 anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) PerCP/Cy5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).

  • APC/Cy7 anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) APC/Cy7 (filled histogram) or mouse IgG2a, κ APC/Cy7 isotype control (open histogram).

  • PE/Cy7 anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1 IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) PE/Cy7 (filled histogram) or mouse IgG2a, κ PE/Cy7 isotype control (open histogram).

  • Brilliant Violet 421™ anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein and then stained with anti-human IgG Fc (clone HP6017) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

  • Alexa Fluor® 488 anti-human IgG Fc
    Human DLL4-transfected CHO cells were

    Human DLL4-transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).

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