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Purified anti-human IgG Fc Antibody
Purified anti-human IgG Fc Antibody
409301 50 µg $65.00 Qty:
    
409302 500 µg $125.00 Qty:
    

Product Details

 
Clone: HP6017 (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control:Purified Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human, Cross-Reactivity: Goat, Rabbit, Sheep, Horse
Immunogen: Purified human immunoglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Clone HP6017 reacts with IgG (Fc fragment) of all subclasses. This antibody does not react with IgA, IgM, purified light chains, or the Fab fragment of IgG.

Application
References:

  Publication Library
Purified anti-human IgG Fc Antibody HP6017 Image 1
Human DLL4 transfected CHO cells were incubated with (top) or without (bottom) recombinant human Notch 1-IgG Fc fusion protein and then stained with human IgG Fc (clone HP6017) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).

Purified anti-human IgG Fc Antibody HP6017 Image 2


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Structure: Homodimer formed by the constant region of the IgG heavy chain
Ligand Receptor: CD16, CD32, CD64
Antigen
References:

1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.

GeneID: 3500
Latest Publications: View the latest IgG Fc articles on HighwirePress.com
UniProt: View information about IgG Fc on UniProt.org
Keywords: Purified anti-human IgG Fc, HP6017, Purified, Human, Cross-Reactivity: Goat, Rabbit, Sheep, Horse, Flow Cytometry, Immunology, Antibodies


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Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 647 anti-human IgG FcHP6017FC
APC anti-human IgG FcHP6017FC
APC/Cy7 anti-human IgG FcHP6017FC
Biotin anti-human IgG FcHP6017FC
Brilliant Violet 421™ anti-human IgG FcHP6017FC
FITC anti-human IgG FcHP6017FC
PE anti-human IgG FcHP6017FC
PE/Cy7 anti-human IgG FcHP6017FC
PerCP/Cy5.5 anti-human IgG FcHP6017FC


Related Pathways

Pathway


Related Categories

Category
Secondary Reagents > anti-human IgG Fc > HP6017
Immunology > Human Immunology > CD and Related Molecules > IgG Fc > HP6017
Immunology > Human Immunology > B Cells > IgG Fc

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) biotin (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by Sav-PE.

  • APC anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).

  • Alexa Fluor® 647 anti-human IgG Fc
    Human DLL4-transfected CHO cells were

    Human DLL4-transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).

  • Purified anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with (top) or without (bottom) recombinant human Notch 1-IgG Fc fusion protein and then stained with human IgG Fc (clone HP6017) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).





  • PE anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with (top) or without (bottom) recombinant human Notch 1-IgG Fc fusion protein and then stained with human IgG Fc (clone HP6017) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).





  • FITC anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).

  • PerCP/Cy5.5 anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) PerCP/Cy5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).

  • APC/Cy7 anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) APC/Cy7 (filled histogram) or mouse IgG2a, κ APC/Cy7 isotype control (open histogram).

  • PE/Cy7 anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1 IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) PE/Cy7 (filled histogram) or mouse IgG2a, κ PE/Cy7 isotype control (open histogram).

  • Brilliant Violet 421™ anti-human IgG Fc
    Human DLL4 transfected CHO cells

    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein and then stained with anti-human IgG Fc (clone HP6017) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

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