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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308506).
Application References:
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 2. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB) 3. Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB) 4. Joseph TD, et al. 2001. J. Biol. Chem. 276:47136. 5. Choi JC, et al. 2007. J. Immunol. 178:1598.
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.
Other Names:
IFN-γRβ, IFNγAF-1
Structure:
Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
Distribution:
All cells, except erythrocytes, at low levels
Function:
Transducing signals
Ligand Receptor:
IFN-γ
Antigen References:
1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305. 2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human IFN-γ R β chain
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
Purified anti-human IFN-γ R β chain
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
