Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308506).
Application References:
1. Kishimoto, T., et al., Eds. 1997. Leucocyte Typing VI. Garland Publishing, Inc. London. 2. Bach, E. A., et al., 1996. Mol. Cell. Biol. 16:3214. 3. Rosenzweig, S. D., et al., 2004. J. Immunol. 173:4000. 4. Joseph, T. D., et al., 2001. J. Biol. Chem. 276:47136. 5. Choi, J. C., et al., 2007. J. Immunol. 178:1598.
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
Description:
IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.
Other Names:
IFN-γRβ, IFNγAF-1
Structure:
Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
Distribution:
All cells, except erythrocytes, at low levels
Function:
Transducing signals
Ligand Receptor:
IFN-γ
Antigen References:
1. Basler, C., et al., 2002. Int. Rev. Immunol. 21:305. 2. Brierley, M., et al., 2002. J. Interferon Cytokine Res. 22:835.
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