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Purified anti-human IFN-γ R β chain Antibody
Purified anti-human IFN-γ R β chain Antibody
308502 100 µg $175.00     

Product Details

Clone: 2HUB-159
Isotype: Hamster IgG
Isotype Control:Purified Armenian Hamster IgG Isotype Ctrl
Workshop
Number:
VI C-111
Reactivity: Human
Immunogen: soluable extracellular domains of the human IFN-γ R β chain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application:

FC - Quality tested
WB, IP - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308506).

Application
References:

1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
2. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB)
3. Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB)
4. Joseph TD, et al. 2001. J. Biol. Chem. 276:47136.
5. Choi JC, et al. 2007. J. Immunol. 178:1598.

Human IFN-γ Rβ transfected L929
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE


Compare all formats



Description:

IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.

Other Names: IFN-γRβ, IFNγAF-1
Structure: Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
Distribution: All cells, except erythrocytes, at low levels
Function: Transducing signals
Ligand Receptor: IFN-γ
Antigen
References:

1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.

GeneID: 3459
Latest Publications: View the latest IFN-gamma R beta chain articles on HighwirePress.com
UniProt: View information about IFN-gamma R beta chain on UniProt.org
Keywords: Purified anti-human IFN-γ R β chain, 2HUB-159, Purified, IFN-γRβ, IFNγAF-1, Human, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human IFN-γ R β chain
    Human IFN-γ Rβ transfected L929

    Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE

  • Purified anti-human IFN-γ R β chain
    Human IFN-γ Rβ transfected L929

    Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE

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