Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking7,9,10 of mixed lymphocyte reactions, and immunohistochemical staining4,5 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 307612).
Application References:
1. Brodsky, F. 1984. Immunogenetics 19:179. 2. Robbins, P., et al., 1987. Human Immunol. 18:301. 3. Stites, D., et al., 1986. Clin. Immunol. Immunopathol. 38:161. 4. Warnke, R., et al., 1980. J. Histochem. Cytochem. 28:771. 5. Engleman, E., et al., 1981. PNAS 78:1791. 6. Zipf, T., et al., 1981. Cancer Res. 41:4786. 7. Goodier, M., et al., 2000. J. Immunol. 165: 139. 8. Esser, M. et al., 2001. J. Virol. 75:6173. 9. Kalka-Moll, W. M., et al., 2002. J. Immunol. 169:1649. 10. Wang, R-F., et al., 1999. Science 284:1351. 11. Davel, AP., et al. 2008.Am J Physiol heart Circ Physiol. 295:H211. PubMed 12. Nagamatsu, T., et al. 2009. Hum Reprod. PubMed 13. Charles, N., et al., 2010. Nat. Med. 16:701. (FC) PubMed
Human peripheral blood lymphocytes stained
with L243 PE
Description:
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. L243 has been reported to block mixed lymphocyte reactions.
Other Names:
Major Histocompatibility Class II, MHC class II
Structure:
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD
Distribution:
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function:
Peptide presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Levacher, M., et al., 1990. Clin. Exp. Immunol. 81:177. 2. Terstappen, L., et al., 1990. J. Leuk. Biol. 48:138. 3. Edwards, J., et al., 1985. J. Immunol. 137:490. 4. van Es, A., et al., 1984. Transplantation 37:65. 5. O'Doherty, U, et al., 1994. Immunology 82:487. 6. Thomas, R., et al., 1994. J. Immunol. 153:4016. 7. Grouard, G., et al., 1996. Nature 384:364.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
