The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
FC-Quality Tested IP8, WB8, IHC(Frozen)4,5 * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking7,9,10 of mixed lymphocyte reactions, and immunohistochemical staining4,5 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 307612).
Application References:
1. Brodsky, F. 1984. Immunogenetics 19:179. 2. Robbins, P., et al., 1987. Human Immunol. 18:301. 3. Stites, D., et al., 1986. Clin. Immunol. Immunopathol. 38:161. 4. Warnke, R., et al., 1980. J. Histochem. Cytochem. 28:771. 5. Engleman, E., et al., 1981. PNAS 78:1791. 6. Zipf, T., et al., 1981. Cancer Res. 41:4786. 7. Goodier, M., et al., 2000. J. Immunol. 165: 139. 8. Esser, M. et al., 2001. J. Virol. 75:6173. 9. Kalka-Moll, W. M., et al., 2002. J. Immunol. 169:1649. 10. Wang, R-F., et al., 1999. Science 284:1351. 11. Davel, AP., et al. 2008.Am J Physiol heart Circ Physiol. 295:H211.PubMed 12. Nagamatsu, T., et al. 2009. Hum Reprod. PubMed
Human peripheral blood lymphocytes stained
with L243 PE
Description:
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. L243 has been reported to block mixed lymphocyte reactions.
Other Names:
Major Histocompatibility Class II, MHC class II
Structure:
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD
Distribution:
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function:
Peptide presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Levacher, M., et al., 1990. Clin. Exp. Immunol. 81:177. 2. Terstappen, L., et al., 1990. J. Leuk. Biol. 48:138. 3. Edwards, J., et al., 1985. J. Immunol. 137:490. 4. van Es, A., et al., 1984. Transplantation 37:65. 5. O'Doherty, U, et al., 1994. Immunology 82:487. 6. Thomas, R., et al., 1994. J. Immunol. 153:4016. 7. Grouard, G., et al., 1996. Nature 384:364.
