The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
FC - Quality tested IHC (frozen) - This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Rodriguez-Gago, M., et al., 2001. Transplantation. 72: 503 2. Lehmann, FS., et al., 2002. Am J Physiol Gastrointest Liver Physiol 283: G481-G488. 3. Bordignon, M., et al., 2008. Mol. Med. Rep. 1:485. (IHC)
Human peripheral blood lymphocytes stained with purified B1, followed by anti-mouse IgG FITC
Description:
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. The γ/δ TCR associates with CD3 and is expressed on a subset of T cells found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.
Other Names:
T cell receptor γ/δ, γ/δ TCR, TCR-γ/δ
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function:
Antigen recognition
Ligand Receptor:
Some bacterial or tumor antigens bound MHC class I, CD1 molecule
Antigen References:
1. Lanier, LL., et al., 1987. J. Clin. Immunol. 7: 429 2. Spencer, J., et al., 1989. Eur. J. Immunol. 19: 1335 3. Uyemura, K., et al., 1991. J. Exp. Med. 174: 683 4. Spada, FM., et al. 2000. J. Exp. Med. 191:907
