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Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA or ELISPOT Capture: The purified JKFb-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JKFb-2 antibody (Cat. No. 508701) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-1 antibody reacts with human fibroblast growth factor - basic (FGF-basic). The JKFb-1 antibody reacts with human fibroblast growth factor - basic (FGF-basic).
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