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Purified anti-human Factor H Antibody
Cat. # Size Price  

518401 50 µg $85.00
    
518402 500 µg $275.00
    
Product Details

Clone: C18/3 (See other available formats)
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: Human Complement Factor H
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
WB - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of Factor H recombinant protein ranging from 78 to 5000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
ELISA Capture: The purified C18/3 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated L20/3 antibody (Cat. No. 518504) as the detection antibody.
Application
References:
  Publication Library
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Antigen Details

Description: Factor H, a 155 kD soluble glycoprotein, is a member of the regulators of complement activation family and is a complement control protein. It circulates in human plasma at a concentration of 500–800 mg/ml that provides critical negative regulation to the alternative pathway of complement cascade. Factor H is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets. Overactive factor H may result in reduced complement activity on pathogenic cells, increasing susceptibility to microbial infections. Underactive factor H may result in increased complement activity on healthy host cells, resulting in autoimmune diseases.
Other Names: Complement Factor H
Antigen
References:
1. Ripoche J, et al. 1988. Biochem. J. 249:593.
2. Rodríguez de Córdoba S, et al. 2004. Mol. Immunol. 41:355.
GeneID: 3075
UniProt: View information about Factor H on UniProt.org
Keywords: Purified anti-human Factor H, C18/3, Purified, Complement Factor H , Human, ELISA Capture, Western Blotting, Immunology, Antibodies
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Sandwich ELISA Protocol
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Version: 1 Revision Date: 2012-11-30
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