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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: The DJR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR4/TRAIL-R1 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307206) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307204) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References:
1. Uno K, et al. 2003. Blood 101:3658. 2. Sato K, et al. 2005. J. Immunol. 174:4025. 3. Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed 4. Chen KF, et al. 2011. J Pharmacol Exp Ther. 337:155. PubMed
Human DR4 transfected cells stained with purified DJR1, followed by anti-mouse IgG FITC
DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.
Other Names:
TRAIL-R1, Apo-2, CD261, TNFRSF10A
Structure:
TNFR superfamily, 56 kD
Distribution:
Activated T cells, tumor cells
Function:
Induces apoptosis
Ligand Receptor:
TRAIL
Antigen References:
1. Macfarlane M. 2003. Toxicol. Lett. 139:89. 2. Baetu T, et al. 2002. Cytokine Growth Factor Rev. 13:199. 3. Degli-Esposti M. 1999. J. Leukoc. Biol. 65:535.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD261 (DR4, TRAIL-R1)
Human DR4 transfected cells stained with biotinylated DJR1, followed by Sav-PE
PE anti-human CD261 (DR4, TRAIL-R1)
Human DR4 transfected cells stained with DJR1 PE
Purified anti-human CD261 (DR4, TRAIL-R1)
Human DR4 transfected cells stained with purified DJR1, followed by anti-mouse IgG FITC
