Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: The DJR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR4/TRAIL-R1 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307206) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307204) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References:
1. Uno, K., et al., 2003. Blood 101: 3658. 2. Sato, K., et al., 2005. J. Immunol. 174:4025. 3. Lundqvist, A., et al. 2006. Cancer Res. 66:7317. PubMed
Human DR4 transfected cells stained with purified DJR1, followed by anti-mouse IgG FITC
Description:
DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.
Other Names:
TRAIL-R1, Apo-2, CD261, TNFRSF10A
Structure:
TNFR superfamily, 56 kD
Distribution:
Activated T cells, tumor cells
Function:
Induces apoptosis
Ligand Receptor:
TRAIL
Antigen References:
1. Macfarlane, M. 2003. Toxicol. Lett. 139:89. 2. Baetu, T., et al., 2002. Cytokine Growth Factor Rev. 13:199. 3. Degli-Esposti, M. 1999. J. Leukoc. Biol. 65:535.
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