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Purified Anti-human DcR1 (TRAIL-R3, CD263) Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human DcR1 TRAIL-R3 CD263 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human DcR1 (TRAIL-R3, CD263) Antibody
307001 25 µg $85.00     
307002 100 µg $175.00     
Clone: DJR3
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: Extracellular domain of DcR1-human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application: FC
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: The DJR3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DcR1/TRAIL-R3 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307004) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application
References:
1. Uno, K., et al., 2003. Blood 101: 3658.
2. Sato, K., et al., 2005. J. Immunol. 174:4025.
Human peripheral blood granulocytes stained

Human peripheral blood granulocytes stained with purified DJR3, followed by biotinylated anti-mouse IgG and Sav-PE


Description: DcR1 is a 65 kD GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.
Other Names: TRAIL-R3, TRID, LIT, CD263, TNFRSF10C
Structure: TNFR superfamily, GPI-anchored, truncated death domain, 65 kD
Distribution: Most normal tissues, not tumors
Function: TRAIL decoy, inhibits apoptosis
Ligand Receptor: TRAIL
Antigen
References:
1. Schneider, P., et al., 1997. J. Biol. Chem. 272:25417.
2. Pan, G., et al., 1997. Science 277:815.
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Purified anti-human DR4 (TRAIL-R1, CD261)DJR1FC
Purified anti-human DcR1 (TRAIL-R3, CD263)DJR3FC
Purified anti-human DR3 (TRAMP)JD3FC
Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICC, IF, IHC, IP, WB
APC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Biotin Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC, ELISA, IHC, IF, WB
FITC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
PE Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC,ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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