Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: The DJR3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DcR1/TRAIL-R3 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307004) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References:
1. Uno, K., et al., 2003. Blood 101: 3658. 2. Sato, K., et al., 2005. J. Immunol. 174:4025.
Human peripheral blood granulocytes stained with purified DJR3, followed by biotinylated anti-mouse IgG and Sav-PE
Description:
DcR1 is a 65 kD GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.
Other Names:
TRAIL-R3, TRID, LIT, CD263, TNFRSF10C
Structure:
TNFR superfamily, GPI-anchored, truncated death domain, 65 kD
Distribution:
Most normal tissues, not tumors
Function:
TRAIL decoy, inhibits apoptosis
Ligand Receptor:
TRAIL
Antigen References:
1. Schneider, P., et al., 1997. J. Biol. Chem. 272:25417. 2. Pan, G., et al., 1997. Science 277:815.
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