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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. (FC) 2. Lahoud MH, et al. 2011. J. Immunol. 187:842. (FC)
Human peripheral blood monocytes were stained with purified anti-human CLEC12a (clone 50C1) (filled histogram) or mouse IgG2a isotype control (open histogram), followed by anti-mouse IgG FITC.
CLEC12A, also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family. It is expressed on monocytes, granulocytes, NK cells, and basophils. Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases.
Other Names:
CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A
Structure:
Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution:
Monocytes, granulocytes, NK cells, and basophils
Function:
Negative regulator of granuocyte and monocyte function
Interaction:
PTPN6 and PTPN11
Antigen References:
1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. 2. Chen CH, et al. 2006. Blood 107:1459. 3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CLEC12A
Human peripheral blood monocytes were stained with purified anti-human CLEC12a (clone 50C1) (filled histogram) or mouse IgG2a isotype control (open histogram), followed by anti-mouse IgG FITC.
PE anti-human CLEC12A
Human peripheral blood monocytes were stained with CLEC12A (clone 50C1) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
