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Purified anti-human CD73 (Ecto-5'-nucleotidase) Antibody
Purified anti-human CD73 (Ecto-5'-nucleotidase) Antibody
344002 100 µg $175.00 Qty:
    

Product Details

 
Clone: AD2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
V B-CD73.3
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IF - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Application
References:

  Publication Library
Purified anti-human CD73 (Ecto-5'-nucleotidase) Antibody AD2 Image 1
Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC

Purified anti-human CD73 (Ecto-5'-nucleotidase) Antibody AD2 Image 2
Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Other Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Structure: GPI-linked 5'-nucleotidase, 70 kD
Distribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Antigen
References:

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

GeneID: 4907
Latest Publications: View the latest CD73 articles on HighwirePress.com
UniProt: View information about CD73 on UniProt.org
Keywords: Purified anti-human CD73 (Ecto-5'-nucleotidase), AD2, Purified, Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT , Human, Flow Cytometry, Immunofluorescence microscopy, Immunology, Antibodies


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Related Products

DescriptionCloneApplications
Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICFC, ICC, IF, IHC, IP, WB
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC


Other Formats

DescriptionCloneApplications
APC anti-human CD73 (Ecto-5'-nucleotidase)AD2FC
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase)AD2FC
FITC anti-human CD73 (Ecto-5'-nucleotidase)AD2FC
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase)AD2FC
PE anti-human CD73 (Ecto-5'-nucleotidase)AD2FC
PE/Cy7 anti-human CD73 (Ecto-5'-nucleotidase)AD2FC
PerCP/Cy5.5 anti-human CD73 (Ecto-5'-nucleotidase)AD2FC


Related Pathways

Pathway
Regulatory T Cells
Stem Cells and Development
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > CD and Related Molecules > CD73 > AD2
Immunology > Human Immunology > Adhesion Molecules > CD73 > AD2
Immunology > Human Immunology > Costimulatory Molecules > CD73 > AD2
Immunology > Human Immunology > Dendritic cells > CD73 > AD2
Immunology > Human Immunology > B Cells > CD73 > AD2
Immunology > Human Immunology > Stem Cells > CD73 (Ecto-5'-nucelotidase)
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD73 (Ecto-5'-nucleotidase)

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2014-04-09
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).





  • Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Brilliant Violet 421™ or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





  • Purified anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC

  • PE anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC

  • APC anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).





  • PE/Cy7 anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 Pacific Blue™ and CD73 (clone AD2) PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom).





  • Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom).





  • PerCP/Cy5.5 anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).





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