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Purified anti-human CD73 (Ecto-5'-nucleotidase) Antibody
Cat. # Size Price  

344002 100 µg $175.00
    
Product Details

Clone: AD2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
V B-CD73.3
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IF - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include:immunofluorescence3.
Application
References:
  Publication Library
 Image 1
Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC

 Image 2
Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC


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Antigen Details

Description: CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Other Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Structure: GPI-linked 5'-nucleotidase, 70 kD
Distribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Antigen
References:
1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
GeneID: 4907
UniProt: View information about CD73 on UniProt.org
Keywords: Purified anti-human CD73 (Ecto-5'-nucleotidase), AD2, Purified, Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT , Human, Flow Cytometry, Immunofluorescence microscopy, Immunology, Antibodies
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Cell Surface Immunofluorescence Staining Protocol
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Version: 2 Revision Date: 2014-04-09
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).





  • Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Brilliant Violet 421™ or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





  • Purified anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC

  • PE anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC

  • APC anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).





  • PE/Cy7 anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 Pacific Blue™ and CD73 (clone AD2) PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom).





  • Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom).





  • PerCP/Cy5.5 anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).





  • Biotin anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained CD3 APC and biotinylated CD73 (clone AD2) (top), or biotinylated mouse IgG1, κ isotype control (bottom), followed by Streptavidin PE.





  • PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).





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