Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen and formalin-fixed paraffin-embedded tissue sections.
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
CD67, CGM6, NCA-95
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Cell adhesion, neutrophil activation
1. Kuijpers T, et al. 1993. J. Immunol. 151:4934. 2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD66b
Human peripheral whole blood granulocytes stained with G10F5 FITC
Purified anti-human CD66b
Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC
Pacific Blue™ anti-human CD66b
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
PE anti-human CD66b
Human peripheral blood granulocytes stained with G10F5 PE
PerCP/Cy5.5 anti-human CD66b
Human peripheral blood granulocytes stained with G10F5 PerCP/Cy5.5
Alexa Fluor® 647 anti-human CD66b
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).
Alexa Fluor® 700 anti-human CD66b
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Alexa Fluor® 700 (filled histogram).