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Purified anti-human CD66b Antibody
Purified anti-human CD66b Antibody
305102 100 µg $95.00 Qty:
    

Product Details

 
Clone: G10F5 (See other available formats)
Isotype: Mouse IgM, κ
Isotype Control:Purified Mouse IgM, κ Isotype Ctrl
Workshop
Number:
VI MA81
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: This antibody is at >85% purity.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen and formalin-fixed paraffin-embedded tissue sections.

Application
References:

  Publication Library
Purified anti-human CD66b Antibody G10F5 Image 1
Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Other Names: CD67, CGM6, NCA-95
Structure: Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution: Granulocytes
Function: Cell adhesion, neutrophil activation
Ligand Receptor: CD66c
Antigen
References:

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

GeneID: 1088
Latest Publications: View the latest CD66b articles on HighwirePress.com
UniProt: View information about CD66b on UniProt.org
Keywords: Purified anti-human CD66b, G10F5, Purified, CD67, CGM6, NCA-95, Human, Cross-Reactivity: Chimpanzee, Flow Cytometry, Immunohistochemistry, Immunology, Antibodies


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Cell Staining BufferFC, ICC, ICFC
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Purified Mouse IgM, κ Isotype CtrlMM-30FC, ICFC, ICC, IF


Other Formats

DescriptionCloneApplications
Alexa Fluor® 647 anti-human CD66bG10F5FC
FITC anti-human CD66bG10F5FC
Pacific Blue™ anti-human CD66bG10F5FC
PE anti-human CD66bG10F5FC
PerCP/Cy5.5 anti-human CD66bG10F5FC


Related Pathways

Pathway
Myeloid-Derived Suppressor Cells


Related Categories

Category
Immunology > Human Immunology > Myeloid Derived Suppressor Cells (MDSCs) > CD66b
Immunology > Non-Human Primate Immunology > CD66b
Immunology > Human Immunology > Neutrophils > CD66b
Immunology > Human Immunology > CD and Related Molecules > CD66b (CD67) > G10F5
Immunology > Human Immunology > Stem Cells > CD66b > G10F5
Immunology > Human Immunology > Adhesion Molecules > CD66b > G10F5

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD66b
    Human peripheral whole blood granulocytes

    Human peripheral whole blood granulocytes stained with G10F5 FITC

  • Purified anti-human CD66b
    Human peripheral blood granulocytes stained

    Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC

  • Pacific Blue™ anti-human CD66b
    Human peripheral blood granulocytes were

    Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).

  • PE anti-human CD66b
    Human peripheral blood granulocytes stained

    Human peripheral blood granulocytes stained with G10F5 PE

  • PerCP/Cy5.5 anti-human CD66b
    Human peripheral blood granulocytes stained

    Human peripheral blood granulocytes stained with G10F5 PerCP/Cy5.5

  • Alexa Fluor® 647 anti-human CD66b
    Human peripheral blood granulocytes were

    Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).

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