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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Human colorectal adenocarcinoma cell line HT29 stained with purified ASL-32 conjugated to PE
The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called as CEA or CEACAM5, primarily found on epithelial cells. CD66e binds to CD66a, c and e.
Other Names:
CEACAM1/5/6, BGP, CEA, NCA
Structure:
Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution:
Granulocytes, epithelial cells
Function:
Homophilic and heterophilic adhesion
Ligand Receptor:
CD66a-e, CD62E, galectins
Antigen References:
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with purified ASL-32 conjugated to PE
PE anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with ASL-32 PE
Alexa Fluor® 488 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 stained with ASL-32 Alexa Fluor® 488
