Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro blocking of adhesion of platelets1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 304912).
Application References:
1. Skinner, M., et al., 1991. J. Biol. Chem. 266:5371. 2. Kishimoto, T. , et al., Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 3. Yen, Y-T., et al., 2006. J. Virolo. 80:2684.
Thrombin-activated human peripheral blood platelets stained with purified AK4, followed by anti-mouse IgGs FITC
Description:
CD62P is a 140 kD type I transmembrane glycoprotein also known as P-Selectin, platelet activation-dependent granule membrane protein (PADGEM), or GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62 is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, platelet-neutrophil, and platelet-monocyte interactions.
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