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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications include: immunoprecipitation1, inhibition of cytolytic activity1 and augment of IL-1 release by TE cells2.
Application References:
1. Sanchez-Madrid F, et al. 1982. P. Natl. Acad. Sci. USA 79:7489. 2. Le PT, et al. 1990. J. Immunol. 144:4541.
Human peripheral blood lymphocytes stained with purified TS2/9, followed by anti-mouse IgG FITC
CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of it's cognate ligand, CD2, on the T cell surface. The HCD58 antibody recognizes human CD58 and has been shown to be useful for flow cytometry.
Other Names:
Lymphocyte function-associated antigen 3, LFA-3
Structure:
Immunoglobulin superfamily member, 45-70 kD cell surface protein. Alternative splicing gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored form on cell surface.
Distribution:
Expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, , erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells
Function:
Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia).
Ligand Receptor:
CD2
Antigen References:
1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223. 2. Dustin ML, et al. 1987. Nature 329:846. 3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861. 4. Sanders ME, et al. 1988. J. Immunol. 140:1401.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD58 (LFA-3)
Human peripheral blood lymphocytes stained with purified TS2/9, followed by anti-mouse IgG FITC
PE anti-human CD58 (LFA-3)
Human peripheral blood lymphocytes stained with TS2/9 PE
PE/Cy5 anti-human CD58
Human peripheral blood lymphocytes stained with TS2/9 PE/Cy5
LEAF™ Purified anti-human CD58 (LFA-3)
Human peripheral blood lymphocytes stained with purified TS2/9, followed by anti-mouse IgG FITC
