The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Valentin, H., et al. 1992. Transplantation. 54: 97
Human peripheral blood lymphocytes stained with purified 097, followed by anti-mouse Ig FITC
Description:
CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression.
Other Names:
Cambridge pathology antigen 1 (CAMPATH-1), epididymal secretory protein 1
Male reproductive tract (epididymis, seminal vesicle), T cells, B cells, macrophage/monocyte, eosinophil, erythrocytes, malignant lymphocytes
Function:
Thought to play a role in carrying and orienting carbohydrates. Target for complement-mediated lysis and antibody-dependent cellular cytotoxicity, used as a depletion target for chronic lymphocytic leukemia/lymphoma therapy and immunosuppression.
Antigen References:
1. Leukocyte Typing VI. Kishimoto, T., et al. (Eds.), Garland Publishing Inc. (1997) 2. Xia, M.-Q., et al., 1991. Eur. J. Immunol. 21:1677. 3. Kirchhoff, C. et al., 1993. Mol. Reprod. Dev. 34:8. 4. Xia, M.Q., et al., 1993. Biochem. J. 293:633.
