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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Pattaramalai s, et al. 1996. Exp. Cell Res. 222:281 2. Skubitz AP, et al. 1996. Am. J. Pathol. 148:1445 3. Skubitz AP, et al. 1998. FEBS Lett. 426:386
Human cervical cancer cell line HeLa cells stained with ASC-1 PE
CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD49c (integrin α3)
Human cervical cancer cell line HeLa cells stained with purified ASC-1 conjugated to PE
PE anti-human CD49c (integrin α3)
Human cervical cancer cell line HeLa cells stained with ASC-1 PE
Alexa Fluor® 488 anti-human CD49c (integrin α3)
Human osteosarcoma cell line MG-63 stained with ASC-1 Alexa Fluor® 488
APC anti-human CD49c (integrin α3)
Human cervical cancer cell line HeLa cells stained with ASC-1 APC
