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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Clone MEM-258 recognizes an epitope on the SCR4 (membrane-proximal SCR) domain of CD46. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting (non-reducing).
Application References:
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Human peripheral blood lymphocytes stained with purified MEM-258, followed by anti-mouse IgGs FITC
CD46 is a transmembrane protein also known as complement membrane cofactor protein, MCP, and measles virus receptor. It is widely expressed on leukocytes, platelets, epithelial cells and fibroblasts. Multiple isoforms of CD46 have been reported with molecular weights ranging from 45-75 kD; CD46 binds complement components C3b and C4b and has also been reported to play a role in T cell regulation. In addition to the complement components, CD46 has been shown to interact with moesin, c-Src, tetraspanin 4, c-Yes, and integrin β1. CD46 is also known to be a cellular receptor for human measles virus and human herpes virus-6. Defects in CD46 have been associated with hemolytic-uremic syndrome. The MEM-258 antibody has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
Transmembrane receptor, contains 4 SUSHI domains, multiple isoforms exist ranging in molecular weight from 45-75 kD. The larger isoform 65-75 kD is not expressed on peripheral blood cells.
Distribution:
Widely expressed in leukocytes, platelets, epithelial cells and fibroblasts
Function:
Binds complement components C3B and C4B with cofactor activity for the I-dependent cleavage of these proteins. May also play a role in T cell regulation
1. Dorig RE, et al. 1993. Cell 75:295. 2. Cui W, et al. 1993. J. Immunol. 151:4137. 3. Kemper C, et al. 2003. Nature 421:388. 4. Marie JC, et al. 2002. Nat. Immunol. 3:659.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD46
Human peripheral blood lymphocytes stained with purified MEM-258, followed by anti-mouse IgGs FITC
FITC anti-human CD46
Human peripheral blood lymphocytes stained with MEM-258 FITC
LEAF™ Purified anti-human CD46
Human peripheral blood lymphocytes stained with LEAF™ purified MEM-258, followed by anti-mouse IgG FITC
