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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
Application References:
1. Horejsi V, et al. 1988. Folia Biol. 34:23. 2. Bazil V, et al. 1989. Immunogenetics 29:202. 3. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 4. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 5. Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.
Human peripheral blood lymphocytes stained with purified MEM-55, followed by anti-mouse IgGs FITC
CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.
Structure:
Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
Distribution:
Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, dendritic cells
Function:
Enhances TCR and BCR signaling
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD45RB
Human peripheral blood lymphocytes stained with MEM-55 PE
Purified anti-human CD45RB
Human peripheral blood lymphocytes stained with purified MEM-55, followed by anti-mouse IgGs FITC
